FIG. 5.

Constant exposure to V. fischeri was required to maintain the symbiont-induced changes in actin. A representative experiment showing relative fluorescence of cross-reactive sites to antiactin in the medial ducts of 48-h juvenile light organs is shown. White bars indicate animals that had no exposure to CM. Solid bars indicate animals that were treated continuously with 20 μg of CM/ml in seawater starting at 12 h. Cohorts of animals that had been inoculated with a CM-resistant strain of V. fischeri [ES114(pVO8)] were used to control for effects of CM. For each condition, n = 15; data are the mean ± standard error of the mean. A * indicates a significant difference (analysis of variance followed by a Tukey's pairwise comparison; P < 0.05) between aposymbiotic animals (± CM) and symbiotic animals colonized by either wild type (± CM) or CM-resistant (± CM) strains of V. fischeri.