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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Nat Med. 2008 Mar 2;14(3):258–265. doi: 10.1038/nm1721

Figure 1. A20 controls the maturation and cytokine production of BM-DCs.

Figure 1

a. Q-RT-PCR analysis of A20 mRNA levels of YFP+ LV-siA20-DC and LV-siGFP-DCs after stimulation with LPS for 12 hr. Experiments were repeated twice with similar results. *P < 0.01, vs. siGFP-DC. Schematic diagram of the recombinant lentiviral vector LV-siA20 (top panel) is presented. H1, H1 RNA promoter; cPPT, central polypurine tract sequence; IRES, internal ribosome entry site; wPRE, Woodchuck post-transcriptional regulatory element sequence; and siA20, A20 small hairpin interfering RNA.

b. Surface expression of molecules on CD11c+/YFP+ gated BM-DC 48 h after viral transfection using FACS analysis. Experiments were repeated three times with similar results.

c. ELISA measurement of the levels of cytokines secreted by transfected DCs (5×105 cells/ml) in response to stimulation with LPS (100 ng/ml) for 16h from one representative experiment of three. *P < 0.01, vs. siA20-DC.