Table 1.
Comparative proteomic of ERI components
| Gene | Protein | Structural Description | # Independent Detection (% Peptide Coverage) | ||
|---|---|---|---|---|---|
|
| |||||
| Common DCR-1, ERI-1 and ERI-5 Interactions | ERI-51 | ERI-1(FLAG)2 | DCR-1(HA)2 | ||
| Y38F2AR.1 | ERI-5 (61.6) | Tudor domain | 5/5 (19) | 2/2 (9) | 3/3 (24) |
| F10B5.7 | RRF-3 (201.4) | RdRP | 5/5 (12) | 2/2 (15) | 3/3 (24) |
| D2005.5 | DRH-3 (129.1) | DEAH/D box RNA helicase | 4/5 (18) | 2/2 (19) | 3/3 (35) |
| K12H4.8 | DCR-1 (210.9) | DExH box RNA helicase/RNaseIII | 4/5 (2) | 2/2 (13) | 3/3 (41) |
| T20G5.11 | RDE-4 (43.4) | dsRBD | 1/5 (6) | 2/2 (11) | 3/3 (44) |
| W09B6.3 | ERI-3 (66.4) | Novel | 1/5 (4) | 2/2 (12) | 3/3 (33) |
| T07A9.5b | ERI-1b (67.2) | SAP domain, exonuclease | 1/5 (2) | 2/2 (31) | 2/3 (13) |
| T23G7.5 | PIR-1 (30.1) | RNA phosphatase | 1/5 (7) | 1/2 (8) | 3/3 (47) |
|
| |||||
| Common DCR-1 and ERI-1 Interactions | |||||
|
| |||||
| C26E6.7 | ERI-9 (73.2) | Novel | nd | 2/2 (7) | 3/3 (7) |
| T06A10.3 | (19.9) | Novel | nd | 2/2 (11) | 2/3 (8) |
| B0001.2 | (105.1) | Novel | nd | 2/2 (7) | 2/3 (6) |
| T07D3.7 | ALG-2 (101.6) | Piwi/PAZ domain | nd | 2/2 (5) | 3 W |
| F48F7.1 | ALG-1 (110.9) | Piwi/PAZ domain | nd | 2/2 (4) | 3 W |
1
Immunopurifications of ERI-5 were conducted using a covalently coupled polyclonal matrix. Since peptide coverage varied between independent samples, peptide coverage for the best ERI-5 recovery is used here.
2
Immunoprecipitations conducted on tagged proteins (Flag and HA, as indicated), expressed from null allele-rescuing transgenes(See Duchaine et al. 2006).
3
These two proteins were not detected in embryonic DCR-1 proteomic analyses, but were detected in adult purifications and with embryonic DCR-1 by western blotting (W).