TABLE 1.

P. fluorescens strains and plasmids used in this study

Strain or plasmid	Genotype and relevant characteristicsa	Reference
Strains
CHA0	Wild type from Morens, Switzerland; DAPG+ HCN+	63
CHA5	hcnB::Ω-Hg insertion derivative of CHA0; Hgr DAPG+ HCN−	64
CHA207	Chromosomal hcnA′-′lacZ fusion of CHA0; DAPG+ HCN−	7
CHA630	phlH::Tn5 single-chromosomal-insertion derivative of CHA0; Kmr DAPG− HCN+	52
CHA631	ΔphlA in-frame-chromosomal-deletion derivative of CHA0; DAPG− HCN+	52
Q2-87	Wild type from Washington State; DAPG+ HCN−	62
Q2-87::Tn5-1	phlD::Tn5-1 chromosomal-insertion derivative of Q2-87; Kmr DAPG− HCN−	3
Plasmids
pME3013	pVK100 with an 8-kb genomic fragment of CHA0 containing functional hcnABC genes conferring HCN biosynthesis; Tcr	64
pME6259	pACYC177-pVS1 carrying a lacZ translational fusion to the phlA of CHA0; Tcr	52
pME6261	pACYC177-pVS1 with a 2-kb fragment of CHA0 containing a functional phlA of CHA0 conferring DAPG biosynthesis; Tcr	52
pMON5118	pCP13/B with a 35-kb genomic fragment of Q2-87 containing the DAPG biosynthetic locus conferring DAPG biosynthesis; Tcr	62

^a+, positive for production; −, no production; Hg^r, mecury resistant (20 μg ml⁻¹); Km^r, kanamycin resistant (25 μg ml⁻¹); Tc^r, tetracycline resistant (125 μg ml⁻¹ for P. fluorescens).