Table 3.
Development of sIPSC characteristics in BLA principal neurons from inhibitory cell-specific conditional Fmr1 KO and rescue animals
| Genotype (n) | Amplitude (mean ± SEM pA) | Frequency (mean ± SEM Hz) | Decay constant (mean ± SEM ms) | Zolpidem enhancement (mean ± SEM %) |
|---|---|---|---|---|
| P10 | ||||
| Dlx5/6Cre (6) | 50.2 ± 1.3 | 1.1 ± 0.09 | 31.5 ± 1.4 | 10.7 ± 4.3 |
| Dlx5/6Cre;Fmr1cKO (6) | 48.2 ± 2.8 | 1.4 ± 0.2 | 34.2 ± 1.0 | 12.0 ± 5.2 |
| p = 0.55 | p = 0.24 | p = 0.15 | p = 0.67 | |
| Dlx5/6Cre;Fmr1cON (5) | 35.6 ± 3.7 | 3.5 ± 0.3 | 40.1 ± 2.0 | 4.4 ± 4.1 |
| p = 0.93 | p = 0.0005 | p = 0.009 | p = 0.0522 | |
| P14 | ||||
| Dlx5/6Cre (6) | 37.7 ± 1.5 | 1.6 ± 0.1 | 34.1 ± 1.2 | 19.5 ± 1.2 |
| Dlx5/6Cre;Fmr1cKO (5) | 35.8 ± 3.3 | 3.0 ± 0.4 | 32.8 ± 1.3 | 22.0 ± 3.7 |
| p = 0.616 | p = 0.022 | p = 0.466 | p = 0.674 | |
| Dlx5/6Cre;Fmr1cON (8) | 42.5 ± 2.7 | 4.5 ± 0.3 | 39.7 ± 0.8 | 5.3 ± 4.4 |
| p = 0.200 | p = 0.00002 | p = 0.006 | p = 0.0521 | |
| P21 | ||||
| Dlx5/6Cre (5) | 49.1 ± 2.2 | 3.3 ± 0.2 | 18.0 ± 0.7 | 54.5 ± 7.3 |
| Dlx5/6Cre;Fmr1cKO (6) | 42.4 ± 2.4 | 3.2 ± 0.2 | 19.9 ± 0.7 | 48.0 ± 4.2 |
| p = 0.058 | p = 0.91 | p = 0.073 | p = 0.46 | |
| Dlx5/6Cre;Fmr1cON (9) | 33.5 ± 2.4 | 6.1 ± 0.6 | 27.8 ± 0.7 | 5.9 ± 4.4 |
| p = 0.00018 | p = 0.0013 | p = 1.0 × 10−8 | p = 1.0 × 10−4 |
Development of sIPSC biophysical characteristics in BLA principal neurons from inhibitory cell-specific conditional Fmr1 KO and rescue animals. The mean ± SEM values for amplitude, frequency, decay constant, and zolpidem enhancement are given for sIPSCs recorded from excitatory BLA principal neurons from control Cre driver (Dlx5/6Cre), conditional Fmr1 KO (Dlx5/6Cre;Fmr1cKO), and conditional Fmr1 rescue (Dlx5/6Cre;Fmr1cON) animals. sIPSCs were sampled from animals at ages P10, P14, and P21. Sample sizes for sIPSC amplitude, frequency, and decay constant for each group are given in parentheses in the first column. Student's two-tailed t test performed for conditional Fmr1 KO (Dlx5/6Cre;Fmr1cKO) and conditional Fmr1 rescue (Dlx5/6Cre;Fmr1cON) mice were individually compared with control mice (Dlx5/6Cre). The p values are listed below the averaged value, and p < 0.05 are shown in bold.