Fig. 3.
Dot blot analysis of apoA-I binding to LP S. A and B: 0.5 μg LPS was loaded onto the nitrocellulose membrane followed by addition of 1 μg of apoA-I. Sample analysis was performed in triplicate for each of the apoA-I variants and LPS control. 1) LPS in the absence of apoA-I; 2) WT apoA-I; 3) ac-apoA-I; 4) apoA-IΔ190-243. The spot intensity is shown in panel B (arbitrary units). C: Spot intensity as a function of apoA-I concentration (0.5 μg LPS).
