Table 1.

ANS and tryptophan fluorescence, and circular dichroism analysis of apoA-I and variants in the presence or absence of LPS.

		ANS fluorescence		Tryptophan fluorescence		Circular dichroism
protein	protein:LPS ratio	λmax	intensity	λmax	intensity	α-helical content
WT apoA-I	1:0	478 nm	143 ± 4	348 nm	499 ± 38	59.2 ± 0.4 %
	2:1	469 nm	188 ± 6	349 nm	613 ± 90	42.3 ± 0.1 %
	1:1	462 nm	202 ± 18	350 nm	623 ± 67	40.1 ± 0.3 %
ac-apoA-I	1:0	482 nm	48 ± 4	348 nm	203 ± 26	43.3 ± 1.6 %
	2:1	482 nm	57 ± 4	348 nm	205 ± 32	43.6 ± 1.9 %
	1:1	482 nm	55 ± 2	348 nm	204 ± 24	41.4 ± 0.5%
apoA-I Δ 190-243	1:0	482 nm	63 ± 2	350 nm	469 ± 65	41.9 ± 1.8 %
	2:1	477 nm	102 ± 2	350 nm	553 ± 59	34.4 ± 3.1 %
	1:1	475 nm	134 ± 5	350 nm	540 ± 78	32.7 ± 4.7 %