FIG. 2.

Western blots of APP and its C-terminal peptides and neprilysin from gray matter (GM) and white matter (WM) homogenates. All cases were quantified by scanning densitometry. The dementia pugilistica (DP) cases are represented as: squares=case # 1; diamonds=case # 2; triangles=case # 3. The mean of the DP and non-demented control (NDC) cases is indicated by a horizontal line. Molecular weights in kDa are reported of the left side of each blot. The actin loading control is below each blot. (A) CT20APP antibody demonstrates the 110 kDa APP holoprotein and the two CT-APP fragments CT99 (∼11 kDa) and CT83 (∼9 kDa). (B) The amyloid-β degrading enzyme, neprilysin, is shown at ∼100 kDa. CT, C-terminal; APP, amyloid-beta precursor protein.