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. 2013 Jun 1;30(11):981–997. doi: 10.1089/neu.2012.2699

FIG. 4.

FIG. 4.

Enzyme-linked immunosorbent assay (ELISA) analysis of α-synuclein and tau in the gray matter (GM) and white matter (WM) of dementia puglistica (DP) and non-demented control (NDC) persons. (A) GM and (B) WM α-synuclein. The brain was first homogenized in Tris-buffer and the remaining pellet homogenized in GDFA/GHCl (Fig. 3A). Both ELISAs were run independently and their results added to generate the scatter plots shown in Figures A and B. Therefore, the numbers given in the figure probably reflect the soluble α-synuclein and not the insoluble molecules associated to the Lewy bodies. Notice that the neuropathological analysis only reported Lewy bodies in the substantia nigra of boxer #3 who also had a concurrent neuropathological diagnosis of Parkinson disease. The frontal GM of this person showed an α-synuclein level similar to the other DP and NDC. (C) GM and (D) WM total tau ELISA. The brain was homogenized as described in the Figure 3A. Both ELISAs were run independently and their results added to generate the scatter plots shown in Figures C and D. See Figure 2 for shape codes. GDFA/GHCl, glass-distilled formic acid/5 M guanidine hydrochloride.