FIG. 5.

One-dimensional (1D) and two-dimensional (2D) Western blot analyses of tau in the gray matter (GM) and white matter (WM) of dementia puglistica (DP) and non-demented control (NDC) persons. (A) 1D-Western blots of GM and WM of urea/thiourea/CHAPS soluble homogenates detected with the HT7 tau antibody. Ten μg of total protein was loaded in each lane. See Figure 2 for shape codes and Western blot notes. (B) GM and (C) WM 2D-Western blot patterns (shown in green and red) as revealed by the HT7 tau antibody. A total of 100 μg of total protein was used for each blot. The numbers at the top of the blots relate to the pI, and the molecular weights are indicated at the left vertical axes. The top blots belong to a pool of the three NDC cases. The bottom three blots belong to each of the three DP cases as indicated at the left margin. The diversity of results and patterns shown in these figures are mainly because of the relative solubility of tau and the extreme insolubility of NFT in the used solvents and also because of the different physical partition techniques. Nonetheless, they demonstrate important isoform differences between DP and NDC cases. As a reference for total protein load, the membranes were stained with the MemCode protein stain after transfer. These blots are shown to the right of each HT7 Western blot.