Table 2.
Antibodies Used in Western Blots
| Primary antibody | Antigen specificity or immunogen | Secondary antibody | Company/Catalog # |
|---|---|---|---|
| CT20APP | Last 20 aa of APP | M | Covance/SIG-39152 |
| Neprilysin | Rat neprilysin | R | Millipore/AB5458 |
| Tau (HT7) | Tau aa 159–163 | M | Pierce/MN1000 |
| BDNF/proBDNF | Internal region of BDNF | R | Santa Cruz/sc-546 |
| GFAP | Recombinant GFAP | R | Abcam/ab7260 |
| α-tubulin | Synthetic peptide of human α-tubulin | R | Cell Signaling/2144S |
| β-tubulin | N-terminal synthetic peptide of human β-tubulin | R | Cell Signaling/2128S |
| Kinesin 5A | Synthetic peptide: aa 1007–1027 | R | Pierce/PA1-642 |
| Dynein | cytoplasmic dynein from chicken embryo brain | M | Sigma/D5167 |
| MBP | aa 119–131 | M | Millipore/MAB381 |
| CNPase | Clone 11–5B | M | Sigma/C5922 |
| Myelin PLP | Synthetic C-terminal peptide | Millipore/MAB388 | |
| Actin Ab-5 | Clone C4 | M | BD TransductionLaboratories/A65020 |
| Actin | N-terminus of human α-actin | R | Abcam/Ab37063 |
CT, C-terminal; APP, amyloid-β precursor protein; aa, amino acid; BDNF, brain-derived neurotrophic factor; GFAP, glial acidic fibrillary protein; MBP, myelin basic protein; CNPase, 2'3'-cyclic nucleotide 3'-phosphodiesterase; PLP, proteolipid protein; M, HRP conjugated AffiniPure goat-anti mouse IgG (catalog # 111-035-144, Jackson Laboratory); R, HRP conjugated AffiniPure goat-anti rabbit IgG, (catalog # 111-035-146 Jackson Laboratory); G, HRP conjugated AffiniPure bovine-anti goat IgG (catalog #805-035-180).