Table 3.
Summary of Biochemical Data
| ELISA results | DP Case #1 | DP Case #2 | DP Case #3 | DP mean | NDC Case A | NDC Case B | NDC Case C | NDC mean | Kruskall-Wallis value | p value | Effect size |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Figure 3B | |||||||||||
| GM Tris-soluble total Aβ40 pg/mg total protein | 15 | 19 | 19 | 18 | 27 | 19 | 19 | 22 | 1.26 | 0.26 | 1.09 |
| Figure 3C | |||||||||||
| GM Tris-soluble total Aβ42 pg/mg total protein | 0 | 106 | 0 | — | 0 | 0 | 0 | — | 1.00 | 0.32 | — |
| Figure 3D | |||||||||||
| GM GDFA/GHCl-soluble total Aβ40 pg/mg total protein | 0 | 2040 | 0 | — | 0 | 0 | 230 | — | 0.07 | 0.80 | 0.72 |
| Figure 3E | |||||||||||
| GM GDFA/GHCl-soluble total Aβ42 pg/mg total protein | 0 | 165060 | 0 | — | 0 | 0 | 12100 | — | 0.07 | 0.80 | 0.75 |
| Figure 3F | |||||||||||
| WM GDFA/GHCl-soluble total Aβ42 pg/mg total protein | 0 | 908 | 0 | — | 131 | 0 | 0 | — | 0.07 | 0.80 | 0.69 |
| Figure 4A | |||||||||||
| GM total α-synuclein ng/mg total protein | 574 | 656 | 521 | 584 | 1059 | 816 | 778 | 884 | 3.86 | 0.05 | 2.55 |
| Figure 4B | |||||||||||
| WM total α-synuclein ng/mg total protein | 339 | 246 | 301 | 295 | 436 | 270 | 189 | 298 | 0.05 | 0.83 | 0.03 |
| Figure 4C | |||||||||||
| GM total tau ng/mg total protein | 2830 | 2090 | 1822 | 2247 | 4682 | 3499 | 2721 | 3634 | 2.33 | 0.13 | 1.76 |
| Figure 4D | |||||||||||
| WM total tau ng/mg total protein | 1614 | 1014 | 1280 | 1303 | 3158 | 2143 | 1246 | 2182 | 1.19 | 0.28 | 1.24 |
| Western blot results (optical density) | DP Case #1 | DP Case #2 | DP Case #3 | DP mean | NDC Case A | NDC Case B | NDC Case C | NDC mean | Kruskall-Wallis value | p value | Effect size |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Figure 2A | |||||||||||
| GM CT20APP 110 kDa | 2.650 | 2.181 | 1.926 | 2.252 | 4.839 | 3.263 | 4.711 | 4.271 | 3.86 | 0.05 | 2.99 |
| GM CT20APP 9–11 kDa | 0.899 | 1.492 | 1.169 | 1.187 | 2.114 | 0.998 | 1.691 | 1.601 | 1.19 | 0.28 | 0.91 |
| WM CT20APP 110 kDa | 1.606 | 1.202 | 1.155 | 1.321 | 2.156 | 2.072 | 0.860 | 1.696 | 0.43 | 0.51 | 0.70 |
| WM CT20APP 9–11 kDa | 0.818 | 0.806 | 1.410 | 1.011 | 1.503 | 1.327 | 0.537 | 1.122 | 0.05 | 0.83 | 0.25 |
| Figure 2B | |||||||||||
| GM neprilysin | 0.706 | 0.306 | 0.309 | 0.440 | 0.784 | 0.919 | 0.911 | 0.871 | 3.86 | 0.05 | 2.50 |
| WM neprilysin | 0.997 | 1.274 | 1.290 | 1.187 | 2.713 | 2.885 | 2.041 | 2.546 | 3.86 | 0.05 | 4.00 |
| Figure 5A | |||||||||||
| GM tau | 1.659 | 4.419 | 1.649 | 2.576 | 15.414 | 15.599 | 16.975 | 15.996 | 3.86 | 0.05 | 7.06 |
| WM tau | 0.885 | 4.149 | 0.722 | 1.919 | 6.626 | 11.692 | 4.008 | 7.442 | 2.33 | 0.13 | 1.83 |
| Figure 6A | |||||||||||
| GM BDNF 32 kDa | 3.794 | 2.894 | 1.051 | 2.580 | 2.295 | 2.529 | 2.832 | 2.552 | 0.43 | 0.51 | 0.03 |
| GM BDNF 15 kDa | 0.918 | 1.079 | 0.394 | 0.797 | 1.334 | 1.626 | 1.669 | 1.543 | 3.86 | 0.05 | 2.19 |
| Figure 6B | |||||||||||
| GM GFAP HMW | 1.927 | 2.234 | 1.622 | 1.928 | 4.621 | 1.134 | 4.505 | 3.420 | 0.43 | 0.51 | 1.04 |
| GM GFAP LMW | 2.805 | 5.707 | 3.795 | 4.102 | 0.489 | 0.079 | 0.808 | 0.459 | 3.86 | 0.05 | 3.37 |
| WM GFAP HMW | 2.430 | 1.278 | 1.401 | 1.703 | 2.197 | 1.625 | 1.894 | 1.905 | 0.43 | 0.51 | 1.26 |
| WM GFAP LMW | 3.127 | 3.005 | 2.545 | 2.892 | 2.100 | 0.335 | 0.319 | 0.918 | 3.86 | 0.05 | 2.61 |
| Figure 6C | |||||||||||
| GM α-tubulin | 2.368 | 2.258 | 2.022 | 2.216 | 2.238 | 2.573 | 2.415 | 2.409 | 1.19 | 0.28 | 1.09 |
| WM α-tubulin | 2.595 | 2.416 | 2.360 | 2.457 | 2.908 | 3.165 | 3.359 | 3.144 | 3.86 | 0.05 | 3.71 |
| Figure 6D | |||||||||||
| GM β-tubulin | 1.662 | 1.699 | 1.660 | 1.674 | 1.611 | 1.591 | 1.532 | 1.578 | 3.86 | 0.05 | 2.85 |
| WM β-tubulin | 1.431 | 1.569 | 1.967 | 1.656 | 2.283 | 2.478 | 2.295 | 2.352 | 3.86 | 0.05 | 3.24 |
| Figure 6E | |||||||||||
| GM kinesin 5A | 1.383 | 1.319 | 1.720 | 1.474 | 2.153 | 2.106 | 2.087 | 2.115 | 3.86 | 0.05 | 4.14 |
| WM kinesin 5A | 1.379 | 1.532 | 2.208 | 1.706 | 2.691 | 2.717 | 2.785 | 2.731 | 3.86 | 0.05 | 3.26 |
| Figure 6F | |||||||||||
| GM dynein | 0.000 | 0.000 | 0.000 | 0.000 | 0.575 | 0.643 | 0.940 | 0.719 | 4.36 | 0.04 | — |
| WM dynein | 1.092 | 1.002 | 1.726 | 1.273 | 3.221 | 3.378 | 2.304 | 2.968 | 3.86 | 0.05 | 3.41 |
| Figure 7A | |||||||||||
| WM MBP | 2.106 | 2.304 | 2.539 | 2.316 | 1.701 | 1.324 | 1.703 | 1.576 | 3.86 | 0.05 | 3.36 |
| Figure 7B | |||||||||||
| WM CNPase | 5.594 | 5.893 | 6.047 | 5.845 | 5.849 | 6.153 | 5.468 | 5.823 | 0.05 | 0.83 | 0.10 |
| Figure 7C | |||||||||||
| WM PLP 24 | 1.710 | 1.541 | 1.782 | 1.678 | 1.549 | 1.610 | 2.285 | 1.815 | 0.05 | 0.83 | 0.46 |
| WM PLP 16 kDa | 3.285 | 3.500 | 3.072 | 3.286 | 2.186 | 3.591 | 6.165 | 3.981 | 0.43 | 0.51 | 0.48 |
ELISA, enzyme-linked immunosorbent assay; DP, dementia pugilistica; NDC, non-demented control; GM, gray matter; GDFA/GHCl, glass-distilled formic acid/guanidine hydrochloride; WM, white matter; CT, C-terminal; APP, amyloid-beta precursor protein; BDNF, brain-derived neurotrophic factor; GFAP, glial fibrillary acidic protein; HMW, high molecular weight; LMW, low molecular weight; MBP, myelin basic protein; CNPase, 2'3'-cyclic nucleotide 3'-phosphodiesterase; PLP, proteolipid protein.