Figure 2.

ERP expression comparison between post-natal developing tooth organs and calvariae. (A) Positive immunoreaction (red) for amelogenin (Amel), ameloblastin (Ambn), and enamelin (Enam) in ameloblasts, enamel matrix, bone matrix, and periosteum. Scale = 100 μm. (B) Western blot detection of amelogenin (Amel), ameloblastin (Ambn), and enamelin (Enam) in calvarial bone extracts at post-natal days 3, 10, and 20 and in developing tooth organs at post-natal day 3. Note that, in this Fig., tooth extracts were diluted at a 1:20 ratio (Ambn and Enam) or a 1:40 ratio (Amel) dilution relative to calvarial extracts, for visualization of expression levels on the same blot. (C) Relative ERP mRNA expression levels between developing calvariae and teeth at post-natal days 3 and 10. ΔCt were normalized to β-actin, and ΔΔCt was performed to calculate fold changes in expression values relative to wild-type calvaria. si = stratum intermedium, amel = ameloblasts, en = enamel, de = dentin, od = odontoblasts, p = periosteum, b = bone, ob = osteoblasts.