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. 2013 Jul;92(7):622–628. doi: 10.1177/0022034513487906

Figure 4.

Figure 4.

Bone transcription factor and marker expression and mineralization levels in calvariae and calvarial osteoblasts from ERP mutant mice. Bone marker RT-real-time PCR of calvariae (A,B) from Amel- and Ambn-deficient mice, suture-patency-related gene (C,D), and calvarial osteoblasts from Amel- (E) and Ambn- (F) deficient mice. Msx2 expression was reduced, while Alx4 expression showed different expression levels in calvariae from Amel- (C) and Ambn- (D) deficient mice. Note the significant reduction in bone marker and transcription factor gene expression levels, e.g., Runx2, Sp7, and Ibsp, in calvarial osteoblasts after loss of Amel and Ambn (E,F). (G) In vitro mineralization assay of primary calvarial osteoblasts from Amel- and Ambn-deficient mice compared with those of wild-types. *p < .05.