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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 3;1822(4):607–614. doi: 10.1016/j.bbadis.2011.11.016

Figure 4. Acetyl-lysine enrichment in SHHF failing hear.

Figure 4

Left ventricular protein extract from the SHHF failing heart was immunoprecipitated with an anti-acetyl-lysine antibody, labeled with CyDye, and then subjected to 2D gel electrophoresis. A portion of the 2D gel is shown. Five of the proteins which demonstrated increased lysine acetylation in both models of heart failure were identified on this gel (circled and numbered in white). Green indicates resolved proteins obtained from CyDye labeling. Molecular weight (kDa) markers are displayed on the left side of the gel and pH markers are displayed underneath.