Table 3. Acetyl-lysine enriched protein spots in the SHHF failing heart.
Five of the 12 proteins which demonstrated increased acetylation in heart failure were identified in an acetyl-lysine enrichment by MALDI-TOF/TOF mass spectrometry and Mascot analysis (C.I. > 99%).
| Protein ID | Access ion No. |
Pred. M.W. |
Pred .P.I. |
Peptide Count |
Protein Score |
C.I. % |
Seq. Cov. % |
Spot # |
|---|---|---|---|---|---|---|---|---|
| ATP synthase beta Subunit | gi|1374715 | 51170.6 | 4.92 | 21 | 1270 | 100 | 67.16 | 1 |
| Long-chain specific acyl-CoA dehydrogenase | gi|205145 | 47842.4 | 7.63 | 15 | 276 | 100 | 34.42 | 2 |
| Muscle creatine kinase | gi|6978661 | 42991.8 | 6.58 | 14 | 570 | 100 | 40.16 | 3 |
| Aspartate aminotransferase, cytoplasmic | gi|122065118 | 46399.5 | 6.73 | 12 | 300 | 100 | 31.72 | 4 |
| Sarcomeric mitochondrial creatine kinase | gi|57537 | 47443.3 | 8.64 | 16 | 565 | 100 | 38.66 | 5 |
Pred. M.W. = Predicted Molecular Weight (Daltons); Pred. P.I. = Predicted P.I. (pH); C.I. = Confidence Interval (%); Seq. Cov. = Sequence Coverage (%).