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The CPE sequence controls GluN2A surface expression in dendrites. SEP-GluN2A-3′ UTR or SEP-GluN2A-ΔCPE-3′ UTR along with mCherry (to visualize the entire neuron) were expressed in cultured hippocampal neurons for 18–24 h. Scale bar, 5 μm. Glycine or vehicle was applied for 3 min (orange bar), and the neurons were imaged every 5 min for 30 min (RM-ANOVA, Bonferroni t tests, n = 5–8 cells; *p < 0.009). Data are mean ± SEM.
