Figure 8.
Effects of ML 3000 on IL-1β-induced IL-8 secretion in human gastric adenocarcinoma (AGS) cells. ML 3000 inhibited both baseline (A) and IL-1β-stimulated (B) IL-8 secretion, with IC50s of 0.46 μM (CI = 0.09 – 2.4 μM) and 1.1 μM (CI = 0.52 – 2.2 μM) respectively. The 5-lipoxygenase-specific inhibitor ZD-2138 showed dose-dependent inhibition of baseline AGS cell IL-8 secretion (C), with an IC50 of 58 nM (CI = 15.6 – 218 nM) and minimal effect on AGS cell IL-1β-stimulated IL-8 secretion (D). The data-points show mean ± s.e. from three independent assays in each of which IL-8 concentrations were measured in triplicate.