Figure 7A and 7B.
(A) CD3−/CD16+/CD56+dim/bright subset expansion as determined by flow cytometry. The CD3− lymphocyte population was gated and used as a reference to determine the percentages of CD3−/CD16+/CD56+dim and CD3−/CD16+/CD56+bright expressions of non-adherent UCB MNCs from CTE versus CTCTE versus CTECT after 48 hours in culture with AB/CY versus SF medium alone. Results represent mean ± SEM (n=3); CD56+dim: P <.05, CTE, CTECT, CTCTE AB/CY versus medium alone; CD56+bright: P <.01, CTE, CTECT, CTCTE AB/CY versus medium alone.
(B) NK Cytotoxicity in NOD/SCID Mouse Xenografted with K562 cells. Effect of UCB ex vivo expanded in medium alone versus AB/CY on survival of NOD/SCID mice that received a xenograft of K562 cells. NOD/SCID mice were injected with 10 × 106 human K562 cells 3 days after tumor cell injection; groups of mice (n=10) received intraperitoneal injections of CTECT UCB cells (1 × 107 cells/animal) stimulated with medium alone or CTECT UCB cells (1 × 107 cells/animal) stimulated with AB/CY. Parallel sham injections of sterile PBS served as a control group. Injections of ex vivo expanded UCB cells or PBS continued every 7 days for 14 days. Tumor survival was monitored daily and animals were killed when they become moribund with disseminated tumor burden.
Reprinted from Biol Blood Marrow Transplant, Vol 12, Ayello J, van de Ven C, Fortino W, et al., Characterization of cord blood natural killer and lymphokine activated killer lymphocytes following ex vivo cellular engineering, 608-622, ©2006, with permission from Elsevier.