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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Semin Hematol. 2010 Jan;47(1):22–36. doi: 10.1053/j.seminhematol.2009.10.009

Figure 8A and 8B.

Figure 8A and 8B

(A) Expression of activating c-lectin receptor CD94/NKG2D after ex-vivo expansion of cryopreserved/thawed/re-cryopreserved/re-thawed (CTCT) cord blood (CB) cells cultured for 2 to 7 days in AB/CY as determined by flow cytometry. CD94/NKG2D expression was significantly increased after incubation at 7 days versus 2 days (p <0.001). Results represent mean ± SEM (n=6). Representative dot plot of CD94/NKG2D expression of CB mononuclear cells (MNCs) after 2-7 days in culture. The lymphocyte population was gated and used as a reference to determine the specific subsets. (B) Expression of NK degranulation marker LAMP-1 (CD107a) of cryopreserved/thawed/re-cryopreserved/re-thawed/ex-vivo expanded (CTCTE) cord blood (CB) cells cultured for 2 to 7 days in AB/CY as determined by flow cytometry. Expression of CD107a in CB CTCTE cells was significantly increased (p <0.001) when comparing day 7 to day 2. Results represent mean ±SEM (n=6). Representative dot plot of CD107a expression of CTCTE CB mononuclear cells (MNCs) after 2-7 days in culture. The lymphocyte population was gated and used as a reference to determine the specific subsets.

Reprinted from Exp Hematol, Ayello J, van de Ven C, Cairo E, et al., Characterization of natural killer (NK) and natural killer-like T (NKT) cells derived from ex-vivo expanded and activated cord blood mononuclear cells: Implications for adoptive cellular immunotherapy (ACI), ©2009, with permission from Elsevier.