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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Methods Mol Biol. 2010;638:33–40. doi: 10.1007/978-1-60761-611-5_3

Fig. 1.

Fig. 1

Schematic of KO cassette construction using yeast recombinational cloning techniques. Primers were designed to amplify flanks both upstream and downstream of the ORF. Each primer also contained 29 nucleotides of “common” sequence to overlap with the yeast cloning vector (light grey) or the hygromycin B phosphotransferase (hph) resistance cassette (dark grey).