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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Gastroenterology. 2011 Nov;141(5):1821–1831. doi: 10.1053/j.gastro.2011.05.049

Figure 4. Dynamic changes in RALDH expression in IEC likely mediated by TNF overproduction and RA deficiency.

Figure 4

(A) Quantification of RALDH3 mRNA from IEC fractions from 4- and 20-week-old TNFΔARE mice compared with WT by real time RT-PCR. Mean±SEM, n=7/strain, *P<0.05 (B) Expression of RALDH1, 2 and 3 mRNA transcripts in IEC fractions from 20-week-old mice, Mean±SEM, n=7/strain. *P<0.05. (C) Analysis of RALDH3 mRNA expression from MODE-K cells cultured for 48 h treated in triplicate with 1μM RA, 10ng/ml TNF, 1μM LE540 or 1μM AM580 stimulation or untreated for 24 h followed by treatment with 1 μM LE540 for 24h (NT/LE). Mean±SEM of 4 individual experiments, *P≤0.05, ***P≤0.001.