Figure 4.

SGK3 expression in human breast tumor samples. A-C, A sample with negative SGK3 staining in invasive carcinoma (A, B) and moderate staining in the surrounding non-neoplastic breast epithelium (C). D-F, A sample with strong SGK3 staining in the invasive carcinoma (D, E) and negative staining in the surrounding non-neoplastic breast epithelium (arrow in D, and F). Both cytoplasmic and nuclear staining was seen in the tumor cells. G-I, A sample with strong SGK3 staining in the invasive carcinoma (G, H) with predominantly cytoplasmic staining. The neighboring nonneoplastic breast epithelium had weak to moderate staining (I). J-K, A sample with moderate SGK3 staining in the invasive carcinoma (J, K) and negative staining in the surrounding breast epithelium (arrow in J, and L). The staining in the tumor cells was cytoplasmic. M-N, A sample with weak SGK3 staining in the invasive carcinoma. O-P, Examples of strong SGK3 staining in non-neoplastic breast epithelium. The middle panels represent boxed higher magnification images of the boxed area in the left panel. Non-neoplastic breast epithelium in D and J as indicated by arrows is shown in F and L respectively. * indicates the lumen of the normal gland. Scale bars, 50 μm. Left panel, ×100. Middle and right panels, ×400.