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Primer sequences for RT-PCR
| Gene | Sequence (5′-3′) |
|---|---|
| TNF-α |
F: GTAGCCCACGTCGTAGCAAA |
|
R: CCCTTCTCCAGCTGGGAGAC | |
| iNOS |
F: GCAGAATGTGACCATCATGG |
|
R: ACAACCTTGGTGTTGAAGGC | |
| TGF-β |
F: GAGAGCCCTGGATACCAACTACTG |
|
R: GTGTGTCCAGGCTCCAAATGTAG | |
| MR |
F: GTAGTTCTATCTTCATCTTC |
|
R: AATATAAGACAGTCACATTA | |
| GAPDH |
F: TCCCTCAAGATTGTCAGCAA |
| R: AGATCCACAACGGATACATT |
F Forward primer, R Reverse primer.
