Table 2.
Additional physical assembly methods identified by survey participants
| Physical Assembly Method | N |
|---|---|
| Commercial/Proprietary |
|
| Proprietary method, not specified |
3 |
| GeneArt Seamless Assembly |
2 |
| GeneArt High Order Assembly |
1 |
| Clontech In-Fusion HD cloning kit |
1 |
| Ginkgo assembly method |
1 |
| Invitrogen TOPO cloning |
1 |
| Non-Commercial |
|
| Conventional PCR [10,11] |
10 |
| Yeast in vivo recombinational cloning [12] |
5 |
| Home-brew method, not specified |
4 |
| Restriction-site Associated DNA (RAD) assembly [13] |
2 |
| Anderson 2 antibiotic (2ab) assembly [14] |
1 |
| Inverse PCR [15] |
1 |
| Splicing by Overlap Extension (SOE) [16] |
1 |
| In development |
|
| A new enzymatic, scarless synthesis and assembly technology |
1 |
| Extensions of BioBytes assembly standard [17] | 1 |