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Biotinylation of cell lines. (A) Silver stain of total protein from biotin treated and non-biotin treated cultured normal canine osteoblasts (CnOb) and two validated canine osteosarcoma cell lines (POS and HMPOS). (B) Anti-biotin Western blot of all biotinylated proteins from biotin treated and non-biotin treated CnOb, POS, and HMPOS cell line samples.
