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. 2013 Jun 13;9:116. doi: 10.1186/1746-6148-9-116

Figure 4.

Figure 4

anti-CD44 immunocytochemistry on live cell lines. A. On-cell Western blot image showing binding of anti-CD44 antibody to the surface of live CnOb (normal canine osteoblast cell line), POS and HMPOS (canine osteosarcoma cell lines). B. Semi-quantitative analysis of CD44 density on cell surface indicating increased surface expression in both osteosarcoma cell lines (POS and HMPOS), relative to normal osteoblasts (CnOb). Quantification measures mean fluorescent intensity (MFI) of anti-CD44 staining, normalized to the approximate number of cells (quantified by nuclear stain).