Table 2.
Summary of observations for selected surface proteins
| Protein name | Mass spec | qRT-PCR | Western blot | ICC |
|---|---|---|---|---|
| Thrombospondin-1 |
|
|
|
|
| CnOb |
D |
High |
High |
N/A |
| POS |
ND |
Low |
Mid |
N/A |
| HMPOS |
ND |
Low |
Low |
N/A |
| CD44 |
|
|
|
|
| CnOb |
ND |
High |
Low |
Low |
| POS |
D |
Low |
High |
High |
| HMPOS |
D |
Low |
Mid |
High |
| CYR61 |
|
|
|
|
| CnOb |
D |
High |
High |
N/A |
| POS |
D |
Low |
High |
N/A |
| HMPOS |
D |
Low |
Low |
N/A |
| Plexin B2 |
|
|
|
|
| CnOb |
ND |
Low |
N/A |
N/A |
| POS |
D |
High |
N/A |
N/A |
| HMPOS |
D |
Mid |
N/A |
N/A |
| Notch-2 |
|
|
|
|
| CnOb |
ND |
Low |
N/A |
N/A |
| POS |
D |
Mid |
N/A |
N/A |
| HMPOS | D | High | N/A | N/A |
A summary of observations for a subset of the surface-exposed proteins identified by mass spectrometry in cultured normal canine osteoblasts (CnOb) and two validated canine osteosarcoma cell lines (POS and HMPOS) and the results of their evaluation via secondary techniques (qRT-PCR, Western blot, and immunocytochemistry [ICC]).
D Detected.
ND Not detected.
High, Mid, Low = relative expression levels observed.