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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Nat Protoc. 2012 Jan 5;7(1):171–192. doi: 10.1038/nprot.2011.431

Figure 2. Timeline for the construction of TALE-TFs and TALENs.

Figure 2

Steps for the construction and functional testing of TALE-TFs and TALENs are outlined. TALEs can be constructed and sequence verified in 5 days following a series of ligation and amplification steps. During the construction phase, samples can be stored at −20°C at the end of each step and continued at a later date. After TALE construction, functional validation via qRT-PCR (for TALE-TFs) and Surveyor nuclease assay (for TALENs) can be completed in 2–3 days.