| Component | Amount | Final concentration |
|---|---|---|
| 0.15 M MgCl2 solution | 2 ul | 15 mM |
| Surveyor Nuclease S | 1 ul | 1x |
| Surveyor Enhancer S | 1 ul
|
1x |
| 4 ul | ||
| Re-annealed duplexes from Step 46Aviii | 16 ul | |
|
| ||
| Total | 20 ul | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.