Table 1. Primers used for quantitative PCR.
Transcript | Locus | Forward primer 5′-3′ | Reverse primer 5′-3′ |
AtGAPDH | AT1G13440 | AGGCTGGGATTGCATTGAGCGA | ACACACAAACTCTCGCCGGTGT |
AtRDR1 | AT1G14790 | AAGAGCGGTTCGGGCGTTGA | AGCCGAAGCCTTTGCTGACTCA |
AtPR1 | AT2G14610 | CGAAAGCTCAAGATAGCCCA | AAGGCCCACCAGAGTGTATG |
AtTPS10 | AT2G24210 | CTGGTGGATGGAGACAGGTT | TGAGGCTCTTGGATTTGTCC |
AtRD29A | AT5G52310 | ACCGATTCATCATCCTCTGTCCGAA | ACGTTATCGGGGTCTCGACGTT |
AtPR4 | AT3G04720 | TGTTCTCCGACCAACAACTG | TGGAGCAATAAGCACTCACG |
AtICS1 | AT1G74710 | CTTTTCAGTCCCTCAGGTTG | AGTTCATCATCCCAAGCAAT |
AtICS2 | AT1G18870 | TGCAGTGTGAAGGACAAGAC | GAAGAGTCTCTCAGGCGTGT |
AtGAPDH was used as the ‘housekeeping’ reference transcript. All primers (used at a final concentration of 10 pmol.µl−1) were designed to have an annealing temperature of 57°C.