Figure 3. Reduced liver fibrosis in CCL3^−/− mice after CCl₄ challenge.

Acute liver injury after CCl₄ challenge is associated with augmented hepatic expression of CCL3, while CCL3 after chronic CCl₄ treatment has a tendency toward higher expression (A). Sirius red staining after the CCl₄ treatment (x100 magnification) shows reduced liver fibrosis in CCL3 deficient mice (B). Hydroxyproline contents in the liver (C) and serum level of ALT (D) were significantly decreased in CCL3^−/− mice. Data are expressed as means ± SEM of eight mice per group. *P<0.05, **P<0.01, ***P<0.001.