Figure 1. Response of a panel of bladder cancer cell lines to ionizing radiation.

Plated cells were exposed to ionizing radiation to measure the effects on growth by clonogenic assay, as described in Methods. (A) Based on the gathered results, we were able to classify these cell lines as radiation–sensitive, moderately sensitive and -relatively resistant. (B) The RAD001 IC50 was plotted against the slope of the curve for each cell line in the clonogenic assay when treated with IR.