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. 2013 Jun 17;8(6):e65257. doi: 10.1371/journal.pone.0065257

Figure 2. Ionizing radiation activates AKT by phosphorylation and RAD001 inhibits S6 phosphorylation.

Figure 2

(A) KU7 cells were treated with 4 Gy of ionizing radiation. Cells were lysed 15, 30 and 60 minutes following treatment to analyze AKT activation (p-AKT; upper row) by Western blot. Total levels of AKT are shown in the lower row. (B) KU7 cells were treated with 5 Gy of radiation, 100 nM of RAD001 or both, and lysed. Levels of Akt and S6 phosphorylation were analyzed by Western blot. Total levels of Akt and S6 expression are shown. Similar results were obtained when 253J-BV, UM-UC3, and UM-UC6 were treated with radiation and RAD001 alone and in combination (data not shown).