Figure 4. Effect of RAD001 and ionizing radiation on the cell cycle.

The cell lines were cultured and treated with RAD001 alone, ionizing radiation alone and with the combination of RAD001 and radiation. For the latter series, samples were pre-treated with RAD001 for 6 hours prior to radiation. Cells were fixed and stained for propidium iodide intake at 48 hrs after treatment, and then measurements were performed by flow cytometry. The proportion of cell populations in the different phases of the cell cycle is shown for each cell line by colored bars (G0/G1: Orange/Blue; S: Red and G2: Yellow).