Table 1.

Kinetic parameters determined for YjgB, YahK, and YqhD* corresponds to data from Jarboe (2010) for different substrates

Enzyme	Cofactor	Substrate	K m (mM)	k cat (s−1)	k cat/K m (s−1 mM−1)
YjgB	NADPH	Acetaldehyde	73.4 ± 5.4	332.6 ± 26.1	4.5
	NADPH	Propionaldehyde	19.6 ± 0.8	207.2 ± 2.9	10.6
	NADPH	Glyceraldehyde	n.c.a.	n.c.a.	n.c.a.
	NADPH	Butyraldehyde	2.1 ± 0.09	464.4 ± 24.2	221
	NADPH	Isobutyraldehyde	193.7 ± 27.5	50.8 ± 9.4	0.26
	NADPH	Crotonaldehyde	2.4 ± 0.2	77.6 ± 3.3	32.3
	NADPH	2-Butanone	n.c.a.	n.c.a.	n.c.a.
	NADPH	Glutaraldehyde	5.8 ± 0.5	311.9 ± 14.8	53.8
	NADPH	5-Hydroxyvalerate	59.7 ± 3.5	2.6 ± 0.04	0.04
	NADPH	Hexanaldehyde	0.34 ± 0.03	418.9 ± 15.7	1,232
	NADPH	Benzaldehyde	0.24 ± 0.02	313.0 ± 7.4	1,305
	NADPH	Furfural	0.22 ± 0.02	224.13 ± 26.9	1,018
	NADP+	Butanol	3.5 ± 0.28	13.79 ± 0.6	3.94
	NADP+	1,4-Butanediol	24.1 ± 1.5	12.9 ± 0.4	0.53
	Butanol	NADP+	0.076 ± 0.01	14.37 ± 0.2	189
	Butyraldehyde	NADPH	0.06 ± 0.003	224.1 ± 36.5	3,734
YahK	NADPH	Acetaldehyde	13.3 ± 1.0	11.18 ± 0.7	0.84
	NADPH	Propionaldehyde	10.9 ± 1.0	11.6 ± 0.6	1.1
	NADPH	Glyceraldehyde	4.4 ± 0.4	12.3 ± 1.3	2.8
	NADPH	Butyraldehyde	2.1 ± 0.1	41.6 ± 0.6	19.8
	NADPH	Isobutyraldehyde	2.2 ± 0.2	32.1 ± 1.2	14.6
	NADPH	Crotonaldehyde	3.6 ± 0.4	32.6 ± 2.1	9.1
	NADPH	2-Butanone	n.c.a.	n.c.a.	n.c.a.
	NADPH	Glutaraldehyde	4.1 ± 0.4	13.4 ± 1.5	3.3
	NADPH	5-Hydroxyvalerat	52.6 ± 13.2	0.18 ± 0.02	0.003
	NADPH	Hexanaldehyde	0.37 ± 0.02	18.3 ± 0.6	49.5
	NADPH	Benzaldehyde	0.29 ± 0.02	7.75 ± 0.5	26.7
	NADPH	Furfural	0.135 ± 0.03	12.5 ± 1.9	92.3
	NADP+	Butanol	6.6 ± 0.3	4.7 ± 0.07	0.71
	NADP+	1,4-Butanediol	38.5 ± 2.9	6.7 ± 0.3	0.18
	Butanol	NADP+	0.012 ± 0.001	7.47 ± 0.07	622.0
	Butyraldehyde	NADPH	0.011 ± 0.001	9.8 ± 0.4	894.4
YqhD*	NADPH	Acetaldehyde	30	1.1	0.033
	NADPH	Propanaldehyde	3.3	45	14
	NADPH	Butyraldehyde	0.67	60	87
	NADPH	Isobutyraldehyde	2	1	0.5
	NADPH	Glyceraldehyde	1.4	3.4	2.4
	NADPH	Furfural	9	n/a	n/a
	NADPH		0.008	n/a	n/a

n.c.a. no catalytic activity, n/a not available