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Decreased Gad1 expression in the dorsal spinal cord of Ascl1−/− mice at E12.5 and E13.5. In situ hybridization on transverse sections of control and Ascl1−/− spinal cords at E10.5 (A, B), E12.5 (C, D), and E13.5 (E, F) using an antisense probe specific for Gad1. Scale bars: A, 50 μm; C, E, 100 μm.
