Figure 4.

Identification of expression domains marked by genes downregulated in Ptf1a^−/− and/or Ascl1^−/− mice. A–G_B, In situ hybridization on spinal cords of control, Ptf1a^−/−, and Ascl1^−/− mice at E18.5 using probes specific for pDyn, Kcnip2, Rorb, Tfap2b, Prkcq, Klhl14, and Npy. A_C–G_C, Quantification of the number of neurons expressing the respective gene in Ptf1a^−/− or Ascl1^−/− mice relative to control. Expression of the more superficially expressed genes pDyn (A–A_C), Kcnip2 (B–B_C), and Prkcq (C, C_C) is reduced to <10% of control levels in Ptf1a^−/− and Ascl1^−/− mice. Expression of NPY (E–E_C) and Tfap2b (G–G_C) is almost completely lost in Ptf1a^−/− but only reduced to 50 or 85% in Mash^−/−, respectively. Error bars represent the SD. Scale bar, 200 μm.