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. 2013 Mar 20;2(3):415–430. doi: 10.1002/mbo3.83

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© 2013 Published by John Wiley & Sons Ltd.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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The Q/N-rich region of Lsm4 possesses antiprion activity. (A) The [URE3] loss by pLSM4^91–187. [URE3][rnq⁻] strain NPK302 was transformed with pRS425GPDp-LSM4 (expressing full-length Lsm4), pRS425GPDp-LSM4^1–90 (non-Q/N-rich region; residues 1–90), pRS425GPDp-LSM4^91–187 (Q/N-rich region; residues 91–187), or an empty vector. Transformants were subjected to the colony color assay. (B) Independence of the curing process from mRNA processing/decay. Coding region of Lsm4's Q/N-rich region was deleted from NPK302 to establish a new strain (ND32) where Lsm4's Q/N-rich region is orthogonal to its non-Q/N-rich region. The experiment as in (A) was then conducted using the ND32 strain.