Figure 7.

Many [URE3]-curable proteins tend to possess [PSI⁺]-inducibility. Gray: [URE3]-curing frequency of each Q/N-rich protein. NPK302 [URE3][rnq⁻] strain was transformed with a series of multicopy plasmids expressing one of the 10 Pin⁺ proteins under the GPD promoters (pRS425GPDp-XXX). Transformants were selected on SC-Leu plates and then subjected to the colony color assay. Curing frequency was semiquantitated by the fraction of the red (cured) colonies out of the total colonies on the YPD plate. At least three independent experiments were carried out. Bars denote the standard deviation. Black: [PSI⁺]-induction frequency of each Q/N-rich protein. NPK51 [psi⁻][rnq⁻] strain was cotransformed with a copper-dependent Sup35NM-expressing plasmid (pRS413CUP1p-SUP35NM) and each of the same Q/N-rich protein-expressing plasmids as above. Transformants were selected and cultured to log phase in SC-Leu-His, and then supplemented with CuSO₄ to the final concentration of 50 μmol/L. Cultures were incubated at 30°C for 48 h. After brief wash, cells were spread on the [PSI⁺]-selective SC-Ade plates and nonselective SC plates for induction frequency quantitation. At least three independent experiments were carried out. Bars denote the standard deviation.