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Effect of divalent cations on amidinotransferase (AMDT) activity of AoaA. Enzyme activity was measured in the presence of different concentrations (0.01–5 mmol/L) of the divalent cations indicated in the graph during 30 min. Data are given as means (n = 3) and error bars represent SD. The 100% activity level was 0.47 μmol/min per mg.
