Fig. 2.

Examples of previously published gating strategies using the same representative donor as in Fig. 1. Top panel demonstrating (A) the two gating strategy based on CD16 positive and CD16 negative monocytes, and (B) the phenotypic profile associated with the two subsets. Lower panel demonstrates (C) the gating strategy of three subsets, regCD14, DP and regCD16 based on CD14 and CD16 expression, and (D) the associated surface marker expression profile. Significant p values are from a post hoc one-way ANOVA. Significant differences are indicated with * (p < 0.05) or ** (p < 0.001).