Fig. 4.

Differences between fresh and cryopreserved PBMCs from 9 individuals with an urban background (African: n = 5; Caucasian: n = 4) were compared. (A) Fresh and cryopreserved monocytes as frequency of live gated population. Monocytes from fresh PBMCs show a significantly smaller proportions of monocytes in comparison to cryopreserved PBMCs, measured non-parametrically with Wilcoxon Signed Ranks Test (z = −2.67, p = 0.004). (B) Mean and SEM of proportions of the five subsets in fresh and cryopreserved preparations. 1: regCD14; 2: dpCD14; 3: HLADRhi; 4: dpCD16; 5: regCD16. Open bars: fresh PBMCs; closed bars: cryopreserved PBMCs. There are no significant differences in proportions dependant on preparation method (measured non-parametrically using the Wilcoxon signed ranks test).