Supplementary Fig. 2.

Lack of effect of rapamycin on tau degradation in neurons. Neurons were transfected with each tau construct and after 48-hour expression the cells were treated for 24 hours with 200 nM rapamycin to activate autophagy. (A) Total cell lysates were probed on Western blots using antibodies against tau (green), β-actin (red), and LC3 (green). * Nonspecific band detected by the LC3 antibody. (B) LC3-I/LC3-II was calculated for each condition (mean ± standard error of the mean; n = 3). Rapamycin did not increase LC3-II or reduce the LC3-I/LC3-II ratio as would be expected after activation of autophagy. Abbreviation: LC3, light-chain 3.