Fig. 2.

Microtubule bundling of tau constructs. (A) CHO cells were transfected with WTtau, E18tau, E27tau, or A18tau and tau expression was visualized by EGFP fluorescence. Microtubules were labeled using the monoclonal α-tubulin antibody, DM1A. WTtau and A18tau exhibited a greater ability to bundle microtubules (arrows). Asterisks indicate cells with microtubule bundles in the presence of low amounts of A18tau. Scale bar, 50μm. (B) Quantification of microtubule-bundling in cells expressing high or low amounts of tau. Values shown are mean ± standard error of the mean. ** p < 0.01; *** p < 0.001, Student t test, relative to WTtau. Abbreviations: CHO, Chinese hamster ovary; EGFP, enhanced green fluorescent protein; MT, microtubule.