Fig. 3.

Microtubule binding of wild type and phosphomutant tau. (A) CHO cells transfected with A18tau, WTtau, E18tau, and E27tau were separated into microtubule-bound and unbound fractions and analyzed using Western blot using an antibody to tau (DAKO). (B) Quantification of microtubule-bound/unbound expressed relative to WTtau (mean ± standard error of the mean; n = 3); * p < 0.05, Student t test, relative to WTtau. (C) Coexpression of DsRed-tagged WTtau and EGFP-tagged E27tau in rat cortical neurons. Arrows indicate colocalization of WTtau and E27tau and asterisks indicate individual E27tau particles. Scale bar, 10 μm. Abbreviations: CHO, Chinese hamster ovary; EGFP, enhanced green fluorescent protein; MT, microtubule.