Fig. 4.

Fast axonal transport of tau. (A) WTtau, E18tau, and E27tau exhibit a punctate morphology in axons in transfected neurons. Scale bar, 5 μm. (B) Kinetic parameters of motile tau particles. Rate between pauses, time spent pausing, number of pauses, and longest distance traveled between pauses were calculated for each tau construct (WTtau, n = 154; E18tau, n = 89; E27tau, n = 81). (C) The number of motile particles per axon is shown for each tau construct. (D) The probability of finding 1 or more than 1 motile tau particle moving in an individual axon was calculated from a randomized selection of movies (WTtau, n =12; E18tau, n = 8; E27tau, n = 7). Values shown are mean ± standard error of the mean. * p < 0.05, ** p <0.01, *** p < 0.001, Student t test, relative to WTtau.