Fig. 6.

Inhibiting autophagy in cortical neurons results in differential degradation of tau phospho-mutants. Neurons were transfected with each tau construct and treated with 10 mM 3MA. (A) Cell lysates were analyzed on Western blots probed with antibodies to tau, β-actin, total and phosphorylated (P) ribosomal S6 protein, and LC3. * Nonspecific band detected by the LC3 antibody. (B) EGFPtau accumulation expressed as a ratio of 3MA-treated and untreated neurons, normalized to β-actin. Values shown are mean ± SEM; n = 5–6; 1-way analysis of variance. (C) Endogenous tau accumulation expressed as a ratio of 3MA-treated and untreated neurons, normalized to β-actin. Values shown are mean ± SEM; n = 6. Abbreviations: EGFP, enhanced green fluorescent protein; LC3, light chain 3; SEM, standard error of the mean.