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. 2013 Jul;41(7):1404–1413. doi: 10.1124/dmd.112.050021

Fig. 2.

Fig. 2.

Effect of BCRP and MDR1 expression on the intracellular AR-67 amounts in MDCKII-pcDNA/BCRP and OVCAR-8/NCI/ADR-RES cells. (A) MDCKII-pcDNA/BCRP and (B) OVCAR-8/NCI/ADR-RES cells were incubated with 0.125, 0.25, 0.5, 0.75 and 1 μM of AR-67 lactone for 5 minutes, and the AR-67 intracellular amount was evaluated. The effect of the dual BCRP/MDR1 inhibitor GF120918 was tested by treating (C) MDCKII-pcDNA/BCRP and (D) OVCAR-8/NCI/ADR-RES cells without (open bars) and with (solid bars) GF120918 (5 µM for 1 hour) before incubating with 1 µM AR-67 lactone for 5 minutes. At the end of the incubation periods, the cells were lysed, and the intracellular AR-67 was quantified using HPLC as mentioned in the Materials and Methods section. Data are represented as mean (n = 3) ± S.D. Statistical analysis was performed using unpaired t test, statistical significance for **P < 0.01; ***P < 0.001.