FIG 2 .

EPEC LEE effector mutants have differing abilities to adhere to HT29 cells and differing effects on MSH2 and MLH1 expression. (A) Western blot showing MSH2 expression in HT29 cells cocultured with EPEC strain E2348/69, effector protein mutants of E2348/69, and the ΔespA mutant (which is incapable of effector protein translocation) for 5 h. (B) HT29 cells were cocultured with EPEC E2348/69 (the wild-type [WT] strain), effector protein mutants of E2348/69, and the ΔespA mutant for 5 h, and bacterial attachment and MSH2 levels were quantified and plotted against one another. (C) Aliquots of the infection supernatant medium were diluted and spread onto agar plates to count nonadherent bacteria. The protein levels of MSH2 were plotted against the CFU of bacteria in the supernatant. Each experiment was repeated at least three times, and values are means (indicated by the markers) ± SEM (error bars).